NEFL 抗体
Quick Overview for NEFL 抗体 (ABIN6938040)
抗原
See all NEFL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
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交叉反应 （详细）
- Human. Rat. Pig. Cow. Chicken.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Neurofilament Purified from human brain
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亚型
- IgG1 kappa
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anti-Neurofilament, Light Polypeptide (NEFL) antibody
NEFL 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 400-543) antibodyNEFL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人, 大鼠, Pig, Cow, 小鸡 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-360) antibodyNEFL 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3G2 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) antibodyNEFL 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-284) antibodyNEFL 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 128-271) antibodyNEFL 适用: 人 IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Light Polypeptide (NEFL) (AA 1-543) antibodyNEFL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunofluorescence (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Brain, Neuroblastoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- NEFL (Neurofilament, Light Polypeptide (NEFL))
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别名
- NEFL
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背景
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68 kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68 kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68,Neurofilament (NF-L) (Neuronal Marker)
Cellular localisation: Cytoplasmic -
分子量
- 68kDa
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基因ID
- 4747, 521461
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UniProt
- P07196
抗原
-
