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NEFH 抗体

NEFH 适用: 人 WB, FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal NF421 unconjugated
产品编号 ABIN6938029
发货至: 中国
  • 抗原 See all NEFH 抗体
    NEFH (Neurofilament, Heavy Polypeptide (NEFH))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This NEFH antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
    交叉反应 (详细)
    Human. Mouse. Rat. Pig. Chicken.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Recombinant full-length human neurofilament (NEFH) protein
    克隆位点
    NF421
    亚型
    IgG1 kappa
    Top Product
    Discover our top product NEFH Primary Antibody
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot(1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HEK293 cells, Brain, Neuroblastoma.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    NEFH (Neurofilament, Heavy Polypeptide (NEFH))
    别名
    NEFH (NEFH 产品)
    别名
    NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody
    背景
    NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200,Neurofilament (NF-H) (Neuronal Marker)
    Cellular localisation: Cytoplasmic
    分子量
    200kDa
    基因ID
    4744, 198760
    UniProt
    P12036
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