Nucleolin 抗体
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- 抗原 See all Nucleolin (NCL) 抗体
- Nucleolin (NCL)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nucleolin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This Mouse Monoclonal Antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
- 无交叉反应
- 牛, 小鼠, 大鼠
- 交叉反应 （详细）
- Human. Does not react with mouse, rat or cow.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human NCL protein
- 克隆位点
- NCL-902
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NCL Primary Antibody
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anti-Nucleolin (NCL) (AA 353-568) antibody
NCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (AA 388-569) antibodyNCL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS, ISt 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 364-5-NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Cat, Gerbil WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 豚鼠, 小鸡, Cat, Gerbil WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (N-Term) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 兔, 马, Saccharomyces cerevisiae, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) (C-Term) antibodyNCL 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: All human cells. Human testis, ovary, uterus, endometrial or Hodgkin s lymphoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Nucleolin (NCL)
- 别名
- NCL (NCL 产品)
- 背景
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NCL, Nucl, Nucleolin, Protein C23,Nucleolin (Marker of Human Cells)
Cellular localisation: Nucleoli - 分子量
- 76kDa
- 基因ID
- 4691, 79110
- UniProt
- P19338
- 途径
- Ribonucleoprotein Complex Subunit Organization
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