NAPSA 抗体 (AA 189-299)
Quick Overview for NAPSA 抗体 (AA 189-299) (ABIN6938010)
抗原
See all NAPSA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
 - AA 189-299
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特异性
 - Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
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交叉反应 (详细)
 - Human.
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纯化方法
 - 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
 - Recombinant human Napsin-A protein fragment (around aa189-299) (exact sequence is proprietary)
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亚型
 - IgG1 kappa
 
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Lung adenocarcinoma.
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限制
 - 仅限研究用
 
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浓度
 - 1.0 mg/mL
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缓冲液
 - Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
 - Azide free
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储存条件
 - -20 °C,-80 °C
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储存方法
 - Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
 - 24 months
 
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- NAPSA (Napsin A Aspartic Peptidase (NAPSA))
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别名
 - NAPSA
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背景
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                        ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA,Napsin A (Lung Adenocarcinoma Marker)
Cellular localisation: Cytoplasmic - 
                                            
分子量
 - 37kDa
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基因ID
 - 9476, 512843
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UniProt
 - O96009
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途径
 - Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
 
抗原
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