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MUC2 抗体

This anti-MUC2 antibody is a 小鼠 单克隆 antibody detecting MUC2 in IHC, IF, FACS 和 IHC (f). Suitable for 人.
产品编号 ABIN6937947
发货至: 中国

Quick Overview for MUC2 抗体 (ABIN6937947)

抗原

See all MUC2 抗体
MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MUC2 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

MLP-842
  • 特异性

    Recognizes a single glycoprotein of 520 kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon, in about 65 % of colonic carcinomas and about 40 % of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.

    无交叉反应

    大鼠

    交叉反应 (详细)

    Human. Does not react with rat.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment covering tandem repeats of human MUC2 protein (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: LS174T cells. Human small intestine (IHC).

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

    别名

    MUC2

    背景

    Mucin-2, Intestinal mucin-2, Mucin like protein, (MLP), SMUC,MUC2 (Mucin 2)
    Cellular localisation: Cytoplasmic and cell surface

    分子量

    520kDa

    基因ID

    4583, 315

    UniProt

    Q02817
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