MUC2 抗体
Quick Overview for MUC2 抗体 (ABIN6937947)
抗原
See all MUC2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a single glycoprotein of 520 kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium.MUC2 is specifically expressed in goblet cells of the small intestine & colon, in about 65 % of colonic carcinomas and about 40 % of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
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无交叉反应
- 大鼠
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交叉反应 (详细)
- Human. Does not react with rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment covering tandem repeats of human MUC2 protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: LS174T cells. Human small intestine (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))
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别名
- MUC2
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背景
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Mucin-2, Intestinal mucin-2, Mucin like protein, (MLP), SMUC,MUC2 (Mucin 2)
Cellular localisation: Cytoplasmic and cell surface -
分子量
- 520kDa
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基因ID
- 4583, 315
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UniProt
- Q02817
抗原
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