MLANA 抗体
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- 抗原 See all MLANA 抗体
- MLANA (Melan A (MLANA))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MLANA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
- 交叉反应 (详细)
- Human. Mouse. Rat. Dog.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length hMART-1 protein (A103, M2-7C10, M2-9E3)
- 克隆位点
- A103-M2-7C10-M2-9E3
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product MLANA Primary Antibody
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- MLANA (Melan A (MLANA))
- 别名
- MLANA (MLANA 产品)
- 别名
- A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
- 背景
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Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA,MART-1 / Melan-A / MLANA (Melanoma Marker)
Cellular localisation: Cytoplasmic - 分子量
- 20-22kDa (doublet)
- 基因ID
- 2315, 154069
- UniProt
- Q16655
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