MLANA 抗体
Quick Overview for MLANA 抗体 (ABIN6937860)
抗原
See all MLANA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
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交叉反应 （详细）
- Human. Mouse. Rat. Dog.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length hMART-1 protein (A103, M2-7C10, M2-9E3)
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亚型
- IgG2a kappa
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anti-Melan A (MLANA) antibody
MLANA 适用: 人, 小鼠, 大鼠 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1761R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal M2-9E3 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 马 IHC, ISt, StM 宿主: 小鼠 Monoclonal MLANA-1761R unconjugated
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) (AA 1-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal A103 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1409R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 小鼠 Monoclonal rMLANA-788 unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-901M- unconjugated Recombinant Antibody
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F3B7 unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MLANA (Melan A (MLANA))
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别名
- MLANA
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背景
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Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA,MART-1 / Melan-A / MLANA (Melanoma Marker)
Cellular localisation: Cytoplasmic -
分子量
- 20-22kDa (doublet)
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基因ID
- 2315, 154069
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UniProt
- Q16655
抗原
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