MCM7 抗体 (AA 195-319)
-
- 抗原 See all MCM7 抗体
- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
-
抗原表位
- AA 195-319
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This MCM7 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.
- 交叉反应 (详细)
- Human. Dog
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant human MCM7 protein fragment (aa195-319)
- 克隆位点
- MCM7-1466
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product MCM7 Primary Antibody
-
-
- 应用备注
-
Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, HEK-293, A549, Raji or Ramos cells. Tonsil.
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
-
- 抗原
- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
- 别名
- MCM7 (MCM7 产品)
- 背景
-
CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Mini chromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,MCM7 (Proliferation Marker)
Cellular localisation: Nuclear - 分子量
- 88kDa
- 基因ID
- 4176, 438720
- UniProt
- P33993
- 途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
-