LIG1 抗体
Quick Overview for LIG1 抗体 (ABIN6937786)
抗原
See all LIG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.
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交叉反应 （详细）
- Human. Mouse. Rat. Bovine.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Full-length native cow LIG1 protein
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亚型
- IgG1 kappa
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anti-Ligase I, DNA, ATP-Dependent (LIG1) antibody
LIG1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 810-919) antibodyLIG1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal AG12 unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 565-790) antibodyLIG1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody
anti-Ligase I, DNA, ATP-Dependent (LIG1) (Internal Region) antibodyLIG1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-919) antibodyLIG1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 810-920) antibodyLIG1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 10G12 unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (C-Term) antibodyLIG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), ELISA, Immunoprecipitation,Optimal dilution for a specific application should be determined.
Positive_Control: MOLT-4, PC-3 or Jurkat cell lysates.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
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别名
- LIG1
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背景
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DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1,DNA Ligase 1
Cellular localisation: Nucleus. -
分子量
- 133kDa
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基因ID
- 3978, 1770
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UniProt
- P18858
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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