Recombinant Cytokeratin 7 抗体
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北京 101111
Quick Overview for Recombinant Cytokeratin 7 抗体 (ABIN6937720)
抗原
See all Cytokeratin 7 (KRT7) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody
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特异性
- It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
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交叉反应 （详细）
- Human.
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纯化方法
- 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.
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免疫原
- Recombinant full-length human KRT7 protein
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亚型
- IgG1, kappa
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anti-Keratin 7 (KRT7) (AA 251-350) antibody
KRT7 适用: 人, 小鼠 WB, FACS, IHC (p), ELISA, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 91-394) antibodyKRT7 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 394-457) antibodyKRT7 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS 宿主: 小鼠 Monoclonal rOV-TL12-30 unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) (AA 2-469) antibodyKRT7 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠 IHC, IF, FACS 宿主: 小鼠 Monoclonal KRT7-760-OV-TL12-30 unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS 宿主: 兔 Monoclonal KRT7-1499R unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 IHC, IF, FACS 宿主: 小鼠 Monoclonal KRT7-903 unconjugated
anti-Keratin 7 (KRT7) (C-Term) antibodyKRT7 适用: 人 WB, IHC, FACS, ICC, IP 宿主: 兔 Monoclonal BC1 unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) antibodyKD Validated KRT7 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB2830 unconjugated Recombinant Antibody
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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别名
- KRT7
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背景
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CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
cervix or breast., HeLa cells. Carcinoma of ovary, Lung
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分子量
- 55kDa
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基因ID
- 3855, 411501
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UniProt
- P08729
抗原
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