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Recombinant Cytokeratin 7 抗体

This anti-Cytokeratin 7 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 7 in WB, FACS 和 IHC (f). Suitable for 人.
产品编号 ABIN6937720
发货至: 中国

Quick Overview for Recombinant Cytokeratin 7 抗体 (ABIN6937720)

抗原

See all Cytokeratin 7 (KRT7) 抗体
Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 7 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

ROV-TL12-30
  • 特异性

    It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant full-length human KRT7 protein

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

    别名

    KRT7

    背景

    CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7,Cytokeratin 7 (Glandular and Transitional Epithelial Marker)
    Cellular localisation: Cytoplasmic

    分子量

    55kDa

    基因ID

    3855, 411501

    UniProt

    P08729
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