KRT20 抗体 (AA 196-323)
Quick Overview for KRT20 抗体 (AA 196-323) (ABIN6937707)
抗原
See all KRT20 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 196-323
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特异性
- This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
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亚型
- IgG kappa
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HT29 cells. Colon Carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- KRT20 (Keratin 20 (KRT20))
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别名
- KRT20
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背景
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CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)
Cellular localisation: Cytoplasmic -
分子量
- 46kDa
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基因ID
- 54474, 84905
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UniProt
- P35900
抗原
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