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KRT20 抗体 (AA 196-323)

This anti-KRT20 antibody is a 小鼠 单克隆 antibody detecting KRT20 in WB 和 IHC (f). Suitable for 人.
产品编号 ABIN6937702
发货至: 中国

Quick Overview for KRT20 抗体 (AA 196-323) (ABIN6937702)

抗原

See all KRT20 抗体
KRT20 (Keratin 20 (KRT20))

适用

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宿主

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小鼠

克隆类型

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  • 59
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单克隆

标记

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This KRT20 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

KRT20-1991
  • 抗原表位

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    AA 196-323

    特异性

    This MAb recognizes an intermediate filament protein of 46 kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HT29 cells. Colon Carcinoma.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    KRT20 (Keratin 20 (KRT20))

    别名

    KRT20

    背景

    CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)
    Cellular localisation: Cytoplasmic

    分子量

    46kDa

    基因ID

    54474, 84905

    UniProt

    P35900
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