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Cytokeratin 18 抗体

This anti-Cytokeratin 18 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 18 in WB, IF, FACS 和 IHC (f). Suitable for 人.
产品编号 ABIN6937677
发货至: 中国

Quick Overview for Cytokeratin 18 抗体 (ABIN6937677)

抗原

See all Cytokeratin 18 (KRT18) 抗体
Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 18 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

DC10
  • 特异性

    This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

    无交叉反应

    牛, 犬, 雪貂, Hamster, 小鼠, 猪, 大鼠, 绵羊

    交叉反应 (详细)

    Human. Does not react with Mouse, Rat, Ferret, Hamster, Sheep, Cow, Dog or Pig.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    PMC-42 human breast carcinoma cells

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7, HeLa, A431 cells. Breast Cancer.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

    别名

    KRT18

    背景

    Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 18 (KRT18)
    Cellular localisation: Cytoplasmic

    分子量

    45kDa

    基因ID

    3875, 406013

    UniProt

    P05783

    途径

    Apoptosis, Caspase Cascade in Apoptosis
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