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KRT15 抗体

This anti-KRT15 antibody is a 小鼠 单克隆 antibody detecting KRT15 in WB, FACS, IF 和 IHC (f). Suitable for 人.
产品编号 ABIN6937663
发货至: 中国

Quick Overview for KRT15 抗体 (ABIN6937663)

抗原

See all KRT15 抗体
KRT15 (Keratin 15 (KRT15))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This KRT15 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

KRT15-2957
  • 特异性

    Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant full-length human KRT15 protein

    亚型

    IgG2c kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HCT116, HeLa, A431 cells. Skin or Colon.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    KRT15 (Keratin 15 (KRT15))

    别名

    KRT15

    背景

    CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15,Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
    Cellular localisation: Cytoplasmic

    分子量

    52kDa

    基因ID

    3866, 654570

    UniProt

    P19012
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