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Keratin 10 抗体

KRT10 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal LH2 unconjugated
产品编号 ABIN6937641
发货至: 中国
  • 抗原 See all Keratin 10 (KRT10) 抗体
    Keratin 10 (KRT10)
    适用
    • 113
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    宿主
    • 71
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    小鼠
    克隆类型
    • 61
    • 57
    单克隆
    标记
    • 64
    • 10
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    This Keratin 10 antibody is un-conjugated
    应用范围
    • 74
    • 55
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    • 36
    • 34
    • 22
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    • 10
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    Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
    交叉反应 (详细)
    Human. Mouse.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Skin extract of a human Psoriasis patient
    克隆位点
    LH2
    亚型
    IgG1 kappa
    Top Product
    Discover our top product KRT10 Primary Antibody
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    Keratin 10 (KRT10)
    别名
    KRT10 (KRT10 产品)
    别名
    BCIE antibody, BIE antibody, CK10 antibody, EHK antibody, K10 antibody, KPP antibody, D130054E02Rik antibody, K1C1 antibody, Krt-1.10 antibody, Krt1-10 antibody, Ka10 antibody, Ker10 antibody, KRT10 antibody, KRT25 antibody, KRT27 antibody, KRT28 antibody, keratin 10 antibody, keratin 10, type I S homeolog antibody, keratin, type I cytoskeletal 25 antibody, KRT10 antibody, Krt10 antibody, krt10.S antibody, LOC101085587 antibody
    背景
    BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10,Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker)
    Cellular localisation: Cytoplasmic
    分子量
    56.5kDa
    基因ID
    3858, 99936
    UniProt
    P13645
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