Recombinant Involucrin 抗体
Quick Overview for Recombinant Involucrin 抗体 (ABIN6937610)
抗原
See all Involucrin (IVL) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
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无交叉反应
- 小鼠
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交叉反应 （详细）
- Human. Gorilla. Owl monkey. Pig. Dog. Does not react with Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-length Involucrin protein (IVRN)
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亚型
- IgG
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anti-Involucrin (IVL) (AA 461-551) antibody
IVL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 65-180) antibodyIVL 适用: 人 ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 301-460) antibodyIVL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 24-118) antibodyIVL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 36-152) antibodyIVL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 犬, Pig, Gorilla, Owl Monkey IHC, ISt, StM 宿主: 小鼠 Monoclonal rIVRN-827 unconjugated Recombinant Antibody
anti-Involucrin (IVL) antibodyIVL 适用: 人, 犬, Pig, 猴 WB, IHC, ELISA, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal SY5 unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 犬, Pig, Gorilla, Owl Monkey IHC, ISt, StM 宿主: 兔 Monoclonal IVRN-2113R unconjugated Recombinant Antibody
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT), (Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37'C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking); Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Involucrin (IVL)
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别名
- IVL
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背景
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IVL,Involucrin (Squamous Cell Terminal Differentiation Marker)
Cellular localisation: Cytoplasmic -
分子量
- 66-170kDa
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基因ID
- 3713, 516439
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UniProt
- P07476
抗原
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