Involucrin 抗体
-
- 抗原 See all Involucrin (IVL) 抗体
- Involucrin (IVL)
-
适用
- 人, Pig, 犬, Gorilla, Owl Monkey
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Involucrin antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
- 无交叉反应
- 小鼠
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Purified involucrin from human keratinocytes
- 克隆位点
- SPM259
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IVL Primary Antibody
-
anti-Involucrin (IVL) (AA 461-551) antibody
IVL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Involucrin (IVL) (AA 65-180) antibodyIVL 适用: 人 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 301-460) antibodyIVL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 24-118) antibodyIVL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 36-152) antibodyIVL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, Pig, 犬, Gorilla, Owl Monkey IHC, ISt, StM 宿主: 小鼠 Monoclonal rIVRN-827 unconjugated Recombinant Antibody
-
- 应用备注
-
Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
-
