ICAM1 抗体
Quick Overview for ICAM1 抗体 (ABIN6937462)
抗原
See all ICAM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Recognizes an 85-115 kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of Purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
-
交叉反应 （详细）
- Human.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Raji Burkitt lymphoma cells
-
亚型
- IgG2b kappa
-
-
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 201-300) antibody
ICAM1 适用: 人, 小鼠, 大鼠, 兔 WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 198-537) antibodyICAM1 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) antibodyICAM1 适用: 人, 大鼠, Cow WB, FACS, ELISA, ICC, IHC (p) 宿主: 小鼠 Monoclonal MEM-111 unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 102-306) antibodyICAM1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 41-464) antibodyICAM1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 18# unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (pTyr512) antibodyICAM1 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 41-464) antibodyICAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 27-209) antibodyICAM1 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 313-327) antibodyVerified ICAM1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 1 (ICAM1) (AA 1-532) antibodyICAM1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3H8-2G6 unconjugated
-
-
应用备注
-
Known_Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
-
别名
- ICAM1
-
背景
-
CD54, Cell surface glycoprotein P3.58, Human rhinovirus receptor, ICAM-1, Intercellular adhesion molecule 1, Ly 47, Major group rhinovirus receptor, MALA2, MyD10, Surface antigen of activated B cells,CD54 / ICAM-1
Cellular localisation: Cell Surface -
分子量
- 85-115kDa
-
基因ID
- 3383, 643447
-
UniProt
- P05362
-
途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
抗原
-
