GPI 抗体
Quick Overview for GPI 抗体 (ABIN6937345)
抗原
See all GPI 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-length GPI protein
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亚型
- IgG2a kappa
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: T47D, PC3, MCF-7, HepG2 and A549 cells. Brain, pancreas or thyroid carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- GPI (Glucose-6-Phosphate Isomerase (GPI))
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别名
- GPI
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背景
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AMF, aurocrine motility factor, EC 5.2.1.9, GNPI, Gpi1, hexose monophosphate isomerase, neuroleukin, NLK, oxoisomerase, PGI, PHI, phosphoglucose isomerase, phosphosaccharomutase, SA-36, sperm antigen 36,Glucose 6-Phosphate Isomerase
Cellular localisation: Cytoplasm. Secreted. -
分子量
- 55kDa
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基因ID
- 2821, 466471
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UniProt
- P06744
抗原
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