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GPI 抗体

The 小鼠 单克隆 anti-GPI antibody (Clone CPTC-GPI-1) (ABIN6937345) specifically detects GPI in WB, IF 和 IHC (f). The antibody is reactive with 人 samples.
产品编号 ABIN6937345
发货至: 中国
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Quick Overview for GPI 抗体 (ABIN6937345)

抗原

See all GPI 抗体
GPI (Glucose-6-Phosphate Isomerase (GPI))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This GPI antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

CPTC-GPI-1
  • 特异性

    Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant human full-length GPI protein

    亚型

    IgG2a kappa
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: T47D, PC3, MCF-7, HepG2 and A549 cells. Brain, pancreas or thyroid carcinoma.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    GPI (Glucose-6-Phosphate Isomerase (GPI))

    别名

    GPI

    背景

    AMF, aurocrine motility factor, EC 5.2.1.9, GNPI, Gpi1, hexose monophosphate isomerase, neuroleukin, NLK, oxoisomerase, PGI, PHI, phosphoglucose isomerase, phosphosaccharomutase, SA-36, sperm antigen 36,Glucose 6-Phosphate Isomerase
    Cellular localisation: Cytoplasm. Secreted.

    分子量

    55kDa

    基因ID

    2821, 466471

    UniProt

    P06744
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