FTL 抗体 (AA 38-165)
Quick Overview for FTL 抗体 (AA 38-165) (ABIN6937273)
抗原
See all FTL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 38-165
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特异性
- Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa 38-165) of human FTL protein (exact sequence is proprietary)
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亚型
- IgG2b kappa
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应用备注
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Known_Application: ELISA (For coating, order Ab without BSA),Western Blot (1-2 μg/mL), Immunohistology (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
Positive_Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Kidney, Cerebellum or Testis.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- FTL (Ferritin, Light Polypeptide (FTL))
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别名
- FTL
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背景
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Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3,Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker)
Cellular localisation: Cytoplasmic -
分子量
- 19-25kDa
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基因ID
- 2512, 433670
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UniProt
- P02792
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途径
- Transition Metal Ion Homeostasis
抗原
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