EPO 抗体 (AA 28-162)
Quick Overview for EPO 抗体 (AA 28-162) (ABIN6937155)
抗原
See all EPO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 28-162
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特异性
- Recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa 28-162) of human EPO protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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anti-Erythropoietin (EPO) (AA 28-193) antibody
EPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 101-193) antibodyEPO 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro), DB 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-193) antibodyEPO 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Erythropoietin (EPO) antibodyEPO 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-162) antibodyEPO 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal EPO-1368 unconjugated
anti-Erythropoietin (EPO) (AA 28-162) antibodyEPO 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal EPO-1367 unconjugated
anti-Erythropoietin (EPO) (AA 20-48) antibodyEPO 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-191) antibodyEPO 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) antibodyEPO 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-193) antibodyEPO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G7 unconjugated
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应用备注
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 cells. Heart or Kidney.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- EPO (Erythropoietin (EPO))
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别名
- EPO
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物质类
- Hormone
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背景
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EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2,Erythropoietin (EPO) (Marker of Placentation Disorders)
Cellular localisation: Cytoplasmic and Cell Surface -
分子量
- 37kDa
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基因ID
- 2056, 2303
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UniProt
- P01588
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途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
抗原
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