EPO 抗体 (AA 28-162)
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- 抗原 See all EPO 抗体
- EPO (Erythropoietin (EPO))
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抗原表位
- AA 28-162
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EPO antibody is un-conjugated
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应用范围
- ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant fragment (around aa 28-162) of human EPO protein (exact sequence is proprietary)
- 克隆位点
- EPO-1367
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product EPO Primary Antibody
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- 应用备注
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 cells. Heart or Kidney.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- EPO (Erythropoietin (EPO))
- 别名
- EPO (EPO 产品)
- 别名
- EPO antibody, EP antibody, MVCD2 antibody, erythropoietin antibody, erythropoietin S homeolog antibody, erythropoietin a antibody, EPO antibody, epo antibody, epo.S antibody, Epo antibody, epoa antibody
- 物质类
- Hormone
- 背景
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EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2,Erythropoietin (EPO) (Marker of Placentation Disorders)
Cellular localisation: Cytoplasmic and Cell Surface - 分子量
- 37kDa
- 基因ID
- 2056, 2303
- UniProt
- P01588
- 途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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