Keratin 5,6,18 抗体
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北京 101111
Quick Overview for Keratin 5,6,18 抗体 (ABIN6937074)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- It recognizes polypeptides of 58 kDa, 56 kDa, and 45 kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
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纯化方法
- Purified by Protein A/G
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免疫原
- Detergent-insoluble fraction of psoriatic human epidermis
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亚型
- IgG1 kappa
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anti-Keratin 5,6,18 antibody
适用: 人, 大鼠, Cow IHC, StM 宿主: 小鼠 Monoclonal LP34 unconjugated
anti-Keratin 5,6,18 antibody适用: 人, 大鼠, Cow IHC, StM, WB, FACS, IF 宿主: 小鼠 Monoclonal SPM267 unconjugated
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应用备注
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Positive Control: HeLa, HepG2 or PC12 cells. Tonsil or Skin.
Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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缓冲液
- 10 mM PBS without BSA and without Azide.
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储存液
- Azide free
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
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有效期
- 24 months
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- Keratin 5,6,18
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别名
- Cytokeratin 5/6/18
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分子量
- 45-58kDa
抗原
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