Keratin 5,6,18 抗体
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- 抗原
- Keratin 5,6,18
- 适用
- 人, 大鼠, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- 特异性
- It recognizes polypeptides of 58 kDa, 56 kDa, and 45 kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Detergent-insoluble fraction of psoriatic human epidermis
- 克隆位点
- SPM267
- 亚型
- IgG1 kappa
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anti-Keratin 5,6,18 antibody
适用: 人, 大鼠, Cow IHC, StM 宿主: 小鼠 Monoclonal LP34 unconjugated
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- 应用备注
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Positive Control: HeLa, HepG2 or PC12 cells. Tonsil or Skin.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Keratin 5,6,18
- 别名
- Cytokeratin 5/6/18
- 分子量
- 45-58kDa
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