CKB 抗体
Quick Overview for CKB 抗体 (ABIN6936996)
抗原
See all CKB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The specificity of this monoclonal antibody to its intended target was validated by HuProt Array containing more than 19,000 full-length, correctly-folded human proteins. Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins.
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交叉反应 (详细)
- Human. Predicted to react with Chimpanzee, Rhesus Monkey, Dog, Cow, Mouse, Rat, Chicken, Zebrafish, Frog.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Human CKBB protein
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亚型
- IgG1 kappa
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应用备注
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Known_Application: ELISA (For coating, order Ab without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Frozen Only) (1-2 μg/mL for 30 minutes at RT) Optimal dilution for a specific application should be determined.
Positive_Control: Cerebellum.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CKB (Creatine Kinase, Brain (CKB))
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别名
- CKB
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背景
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B-CK, BB-CK, creatine kinase-B, Creatine kinase B chain, CKBB creatine kinase B-type, creatine kinase, brain, CKBB, Brain creatine kinase, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine kinase brain, Creatine phosphokinase BB,Creatine Kinase-BB (CK-BB)
Cellular localisation: Cytoplasmic -
分子量
- 43kDa (Monomer), 86kDa (Dimer)
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基因ID
- 1152, 173724
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UniProt
- P12277
抗原
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