CKB 抗体
Quick Overview for CKB 抗体 (ABIN6936995)
抗原
See all CKB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-length CKB protein
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亚型
- IgG2b kappa
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anti-Creatine Kinase, Brain (CKB) antibody
CKB 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 11-367) antibodyCKB 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D3 unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 2-270) antibodyCKB 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 66-179) antibodyCKB 适用: 人, 小鼠 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 7-381) antibodyCKB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) antibodyCKB 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 1-381) antibodyCKB 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3D5-3D11 unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 1-381) antibodyCKB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) antibodyCKB 适用: 人 ELISA, IF, FACS, IHC (fro), Coat 宿主: 小鼠 Monoclonal 2ba6 unconjugated
anti-Creatine Kinase, Brain (CKB) antibodyCKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CPTC-CKB-2 unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: SH-SY5Y or HEK-293 cells. Human cerebellum.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CKB (Creatine Kinase, Brain (CKB))
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别名
- CKB
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背景
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BCK, Brain creatine kinase, Ckb, Creatine kinase B chain, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine phosphokinase BB, Epididymis luminal protein 211, Epididymis secretory protein Li 29, HEL 211,Creatine Phosphokinase-BB (CK-BB)
Cellular localisation: Cytoplasm -
分子量
- 42kDa
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基因ID
- 1152, 173724
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UniProt
- P12277
抗原
-
