Chp2 抗体
Quick Overview for Chp2 抗体 (ABIN6936993)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-length CHP2 protein
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亚型
- IgG2c kappa
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anti-Chp2 antibody
适用: 人 WB, IF 宿主: 小鼠 Monoclonal CPTC-CHP2-1 unconjugated
anti-Chp2 (Internal Region) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chp2 (Internal Region) antibody适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chp2 (Internal Region) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chp2 (Internal Region) antibody适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chp2 (Center) antibody适用: 人, 小鼠, 大鼠, 猴 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Chp2 (AA 101-150) antibody适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, K562 or Jurkat cell lysates. Human appendix or testis.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Chp2
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背景
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Calcineurin-like EF hand protein 2, calcineurin-like EF-hand protein 2, HCA520, hepatocellular carcinoma antigen gene 520, Hepatocellular carcinoma-associated antigen 520 homolog,Calcineurin B homologous protein 2 / HCC Antigen 520
Cellular localisation: Nucleus. Cytoplasm. -
分子量
- 22kDa
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基因ID
- 63928, 178589
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UniProt
- O43745
抗原
-
