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CDC20 抗体

CDC20 适用: 人 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal AR12 unconjugated
产品编号 ABIN6936871
发货至: 中国
  • 抗原 See all CDC20 抗体
    CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
    适用
    • 56
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    宿主
    • 44
    • 13
    小鼠
    克隆类型
    • 43
    • 14
    单克隆
    标记
    • 33
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    This CDC20 antibody is un-conjugated
    应用范围
    • 36
    • 22
    • 20
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    • 12
    • 9
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    • 4
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
    交叉反应 (详细)
    Human.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Urea-denatured His6 Cdc20 human recombinant protein
    克隆位点
    AR12
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CDC20 Primary Antibody
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
    别名
    CDC20 (CDC20 产品)
    别名
    X-FZY antibody, ba276h19.3 antibody, cdc20a antibody, fizzy1 antibody, p55cdc antibody, CDC20A antibody, bA276H19.3 antibody, p55CDC antibody, 2310042N09Rik antibody, C87100 antibody, cell division cycle 20 antibody, cell division cycle 20 L homeolog antibody, cell division cycle 20 homolog (S. cerevisiae) antibody, cdc20 antibody, CDC20 antibody, cdc20.L antibody, Cdc20 antibody
    背景
    CDC20, CDC20A, p55CDC, P55CDC-LSB,Cdc20 (Cell Division Cycle Protein 20)
    Cellular localisation: Cytoplasmic
    分子量
    55kDa
    基因ID
    991, 524947
    UniProt
    Q12834
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