CD99 抗体
Quick Overview for CD99 抗体 (ABIN6936865)
抗原
See all CD99 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
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无交叉反应
- 大鼠
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交叉反应 （详细）
- Human. Does not react with Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human MIC2 protein
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亚型
- IgG1 kappa
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anti-CD99 (CD99) (AA 1-185) antibody
CD99 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7E11C4 unconjugated
anti-CD99 (CD99) (AA 1-185) antibodyCD99 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1B10E12 unconjugated
anti-CD99 (CD99) (AA 23-122) antibodyCD99 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-CD99 (CD99) (AA 23-185) antibodyCD99 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 27-161) antibodyCD99 适用: 小鼠 FACS, WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 26-160) antibodyCD99 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3B2-TA8 unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5C8 unconjugated
anti-CD99 (CD99) antibody (PE)CD99 适用: 人 FACS 宿主: 小鼠 Monoclonal 3B2-TA8 PE
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, WB, IHC, ELISA 宿主: 兔 Monoclonal 9A5 unconjugated Recombinant Antibody
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应用备注
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Known_Application: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at Room Temp)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
Positive_Control: MOLT-4 cells. Pancreas or Ewing's sarcoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD99
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别名
- CD99
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背景
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12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2,CD99 / MIC2 (Ewing's Sarcoma Marker)
Cellular localisation: Cell surface -
分子量
- 27-32kDa
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基因ID
- 4267, 653349
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UniProt
- P14209
抗原
-
