Recombinant CD81 抗体
Quick Overview for Recombinant CD81 抗体 (ABIN6936837)
抗原
See all CD81 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
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交叉反应 (详细)
- Human, Mouse and Rat. Others not known.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- B-Cell line derived from a Burkitt lymphoma
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亚型
- IgG
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应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistology (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, and human lymphocytes. Tonsil or Liver.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD81
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别名
- CD81 / TAPA-1
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背景
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CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28,CD81 / TAPA-1
Cellular localisation: Cell surface -
分子量
- 26kDa
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基因ID
- 975, 54457
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UniProt
- P60033
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途径
- Inositol Metabolic Process, Hepatitis C
抗原
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