CD72 抗体
Quick Overview for CD72 抗体 (ABIN6936804)
抗原
See all CD72 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- CD5 has been identified as a transmembrane glycoprotein that is expressed on 70 % of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/CD3 chain, and with the Src family kinase, Lck p56. The C-type lectin superfamily member CD72 is a cell surface negative regulator of B cell activation from the pro-B through the mature B cell stage. CD72 serves as a receptor for CD5. The ability of lymphocytes to respond to antigenic or mitogenic stimulation utilizes both positive and negative regulatory proteins that influence the threshold for responsiveness. The human CD72 gene maps to chromosome 9p13.3 and encodes a transmembrane glycoprotein that contains an ITIM. Upon tyrosine phosphorylation, the CD72 ITIM recruits SH2-containing phosphatases such as SHP-1, resulting in downregulation of cell activation. CD72-/- mice contain hyperproliferative B cells.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- HF B1 plasmacytoid cell line.
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亚型
- IgG2a kappa
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anti-CD72 Molecule (CD72) antibody
CD72 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal 3F3 unconjugated
anti-CD72 Molecule (CD72) (AA 117-359) antibodyCD72 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 4G4B1 unconjugated
anti-CD72 Molecule (CD72) (AA 117-359) antibodyCD72 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2F3B8 unconjugated
anti-CD72 Molecule (CD72) (AA 169-359) antibodyCD72 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) (AA 143-345) antibodyCD72 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) (AA 126-237) antibodyCD72 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) antibodyCD72 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal BU40 unconjugated
anti-CD72 Molecule (CD72) (AA 4-110) antibodyCD72 适用: 人 FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) (AA 150-208) antibodyCD72 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD72 Molecule (CD72) antibodyCD72 适用: 人 WB, FACS, IP, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal Bu40 unconjugated
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应用备注
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Known_Application: ELISA (For coating, order without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K562 cel lysate. Tonsil or Lymph Node.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD72 (CD72 Molecule (CD72))
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别名
- CD72
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背景
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B cell differentiation antigen CD72, CD72b, CD72c, Ly-19, Ly-32, Ly-m19, LYB2, Lymphocyte antigen 32,CD72 (B Cell Marker)
Cellular localisation: Membrane -
分子量
- 45kDa
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基因ID
- 971, 116481
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UniProt
- P21854
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途径
- BCR Signaling
抗原
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