CD68 抗体
Quick Overview for CD68 抗体 (ABIN6936788)
抗原
See all CD68 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
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无交叉反应
- 小鸡, 犬, 猪
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交叉反应 (详细)
- Human. Monkey. Rabbit. Cat. Does not react with Pig, Dog or Chicken.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Subcellular fraction of human alveolar macrophages
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Tonsil, Lymph Node or Spleen.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD68 (CD68 Molecule (CD68))
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别名
- CD68
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背景
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GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1,CD68 (Macrophage Marker)
Cellular localisation: Cell Surface and Cytoplasmic -
分子量
- 110kDa
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基因ID
- 968, 647419
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UniProt
- P34810
抗原
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