CD63 抗体 (AA 100-197)
Quick Overview for CD63 抗体 (AA 100-197) (ABIN6936780)
抗原
See all CD63 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 100-197
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特异性
- This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
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交叉反应 （详细）
- Human and Mouse. Others not known.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human CD63 protein fragment (around aa100-197) (exact sequence is proprietary)
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亚型
- IgG2b kappa
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anti-CD63 (CD63) (AA 120-175) antibody
CD63 适用: 人, 小鼠, 大鼠, 猴, 犬 FACS, WB, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 unconjugated
anti-CD63 (CD63) antibody (Biotin)CD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 Biotin
anti-CD63 (CD63) antibody (APC)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 APC
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 1H1E11 unconjugated
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 10F11E6 unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人, 小鼠 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal MX-49-129-5 unconjugated
anti-CD63 (CD63) antibody (PerCP)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PerCP
anti-CD63 (CD63) antibody (PE)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PE
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1E8D4 unconjugated
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应用备注
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Known_Application: Western Blotting (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Human spleen or lymphoma tissue (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD63
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别名
- CD63
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背景
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Gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30,CD63 (Late Endosomes Marker)
Cellular localisation: Cell Surface. Cytoplasm. -
分子量
- 26kDa (core protein), 30-60kDa (glycosylated)
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基因ID
- 967, 445570
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UniProt
- P08962
抗原
-
