CD3 epsilon 抗体 (Cytoplasmic Domain)
-
- 抗原 See all CD3 epsilon (CD3E) 抗体
- CD3 epsilon (CD3E)
-
抗原表位
- AA 156-168, Cytoplasmic Domain
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CD3 epsilon antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
- 交叉反应 (详细)
- Human, Rat, Guinea pig, Rabbit, Pig, Horse, Cow and Chicken. Others not known.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain
- 克隆位点
- PC3-188A
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD3E Primary Antibody
-
-
- 应用备注
-
Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blotting (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Tonsil and lymph node
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
-
- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 别名
- cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
- 背景
-
CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3e (T-Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic - 分子量
- 20kDa
- 基因ID
- 916, 3003
- UniProt
- P07766
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
-