Cyclin D1 抗体
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北京 101111
Quick Overview for Cyclin D1 抗体 (ABIN6936590)
抗原
See all Cyclin D1 (CCND1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40 % of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
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交叉反应 （详细）
- Human. Monkey. Mouse. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Human full length recombinant cyclin D1 protein
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亚型
- IgG2a kappa
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anti-Cyclin D1 (CCND1) (AA 61-110) antibody
CCND1 适用: 人, 小鼠, 大鼠, Cow, 兔, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (AA 167-295) antibodyCCND1 适用: 人 IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3B6G3 unconjugated
anti-Cyclin D1 (CCND1) (AA 1-295) antibodyCCND1 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1F7F5 unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) antibodyKD Validated CCND1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB735 unconjugated Recombinant Antibody
anti-Cyclin D1 (CCND1) (AA 68-97) antibodyCCND1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB8066 unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) antibodyCCND1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal DCS-6 unconjugated
anti-Cyclin D1 (CCND1) (AA 1-295) antibodyCCND1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Cyclin D1 (CCND1)
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别名
- CCND1
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背景
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B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene,Cyclin D1 (G1-Cyclin & Mantle Cell Marker)
Cellular localisation: Nuclear -
分子量
- 36kDa
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基因ID
- 595, 523852, 667996
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UniProt
- P24385
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途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
抗原
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