Calpastatin 抗体
Quick Overview for Calpastatin 抗体 (ABIN6936579)
抗原
See all Calpastatin (CAST) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human CAST protein
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亚型
- IgG1 kappa
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anti-Calpastatin (CAST) antibody
CAST 适用: 人 ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal CAST-1550 unconjugated
anti-Calpastatin (CAST) (AA 1-667) antibodyCAST 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 582-669) antibodyCAST 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-Calpastatin (CAST) (AA 275-310) antibodyCAST 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-350) antibodyCAST 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 431-530) antibodyCAST 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 275-700) antibodyCAST 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-686) antibodyCAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-667) antibodyCAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calpastatin (CAST) antibody (FITC)CAST 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
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应用备注
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Esophagus or Small Intestine.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Calpastatin (CAST)
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别名
- CAST
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背景
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BS 17, Calpain inhibitor, Calpastatin, Cast, Heart type calpastatin, Sperm BS 17 component,Calpastatin
Cellular localisation: Cytoplasm and Cell Surface -
分子量
- 126kDa
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基因ID
- 831, 436186
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UniProt
- P20810
抗原
-
