CAPNL1 抗体
Quick Overview for CAPNL1 抗体 (ABIN6936578)
抗原
See all CAPNL1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
-
交叉反应 （详细）
- Human.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant full-length human CAPN1 protein
-
亚型
- IgG1 kappa
-
-
anti-Calpain 1, Large Subunit (CAPNL1) antibody
CAPNL1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 20-330) antibodyCAPNL1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 501-714) antibodyCAPNL1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5B2G10 unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 387-694) antibodyCAPNL1 适用: 大鼠 WB, IHC, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 387-694) antibodyCAPNL1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 501-714) antibodyCAPNL1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1E6G9 unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 312-326), (Middle Region) antibodyCAPNL1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 387-694) antibodyCAPNL1 适用: 小鼠 WB, IHC, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 75-343) antibodyCAPNL1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1, Large Subunit (CAPNL1) (AA 20-330), (Catalytic Subunit) antibodyCAPNL1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Skin or Tonsil.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
-
别名
- CAPN1
-
背景
-
Calcium dependent protease small subunit 1, Calcium-activated neutral proteinase 1, Calpain 1 large subunit, Calpain mu-type, Calpain regulatory subunit, Calpain small subunit 1, Calpain, large polypeptide L1, Calpain-1 catalytic subunit, CANP small subunit, CANP1, CANPL1, CAPN1, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, Mu Calpain,Calpain 1
Cellular localisation: Cytoplasm and Cell Surface -
分子量
- 80kDa
-
基因ID
- 823, 502842
-
UniProt
- P07384
-
途径
- Apoptosis
抗原
-
