C4B 抗体
Quick Overview for C4B 抗体 (ABIN6936545)
抗原
See all C4B (C4b) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
-
交叉反应 （详细）
- Human.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant human Complement 4d protein
-
亚型
- IgG1 kappa
-
-
anti-Complement Component C4b (C4b) (AA 126-366) antibody
C4b 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 680-756) antibodyC4b 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 1347-1446) antibodyC4b 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1F2 unconjugated
anti-Complement Component C4b (C4b) (AA 19-333) antibodyC4b 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 1454-1744) antibodyC4b 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) antibodyC4b 适用: 人 IHC, IF, StM 宿主: 小鼠 Monoclonal SPM545 unconjugated
anti-Complement Component C4b (C4b) (AA 144-193) antibodyC4b 适用: 人, 小鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4b (C4b) (AA 655-684) antibodyC4b 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: Immunofluorescence (1:50-1:100), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Rejected Renal Transplant Tissue.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- C4B (C4b) (Complement Component C4b (C4b))
-
别名
- C4B
-
背景
-
Acidic Complement C4, Basic C4, C4, C4-1, C4a Anaphylatoxin, C4A, C4A2, C4A3, C4A4, C4A6, C4b, C4B1, C4B12, C4d fragment, C4F, C4S, Complement C4 Gamma Chain, Complement Component 4A, Complement Component 4B,Complement 4d (C4d) (Acute Humoral Rejection Marker)
Cellular localisation: Intracytoplasmic vacuoles of endothelial cells and Secreted -
分子量
- 192kDa (predicted)
-
基因ID
- 720, 721, 534847
-
途径
- Complement System
抗原
-
