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Biotin 抗体

Name: Biotin 抗体
Brand: antibodies-online
SKU: ABIN6936505
Availability: OutOfStock

WB, FACS, IHC, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated

产品编号 ABIN6936505

发货至: 中国

Datasheet as PDF

抗原 See all Biotin products

Biotin

适用

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宿主
56

32

28
小鼠
Compatible Secondaries
克隆类型
75

34
单克隆
标记
28

11

8

7

7

4

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is un-conjugated
应用范围
54

48

43

35

32

19

14

12

12

12

9

9

5

4

4

3

2

2

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)

特异性

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

纯化方法

Purified by Protein A/G

免疫原

Biotinylated sheep immunoglobulin

克隆位点

Hyb-8

亚型

IgG1 kappa
anti-Biotin antibody
WB, ELISA, IP, DB 宿主: 兔 Polyclonal unconjugated

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody
ELISA 宿主: 山羊 Polyclonal unconjugated Fab fragment

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA 宿主: 山羊 Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA 宿主: 小鼠 Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl 宿主: 山羊 unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con 宿主: 小鼠 Monoclonal unconjugated

anti-Biotin antibody
IP, Con 宿主: 兔 Polyclonal unconjugated

应用备注

Positive Control: Biotinylated proteins in solution or on tissues.
Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

限制

仅限研究用
缓冲液

10 mM PBS without BSA and without Azide.

储存液

Azide free

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

有效期

24 months
抗原

Biotin

别名

Biotin (Vitamin B7 or Vitamin H) (Biotin 产品)

物质类

Chemical

分子量

244Da