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ARF1 抗体 (AA 174-180)

The 小鼠 单克隆 anti-ARF1 antibody (Clone 3F1) (ABIN6936427) specifically detects ARF1 in WB, ELISA, IF, Coat 和 IHC (f). The antibody is reactive with 人 samples.
产品编号 ABIN6936427
发货至: 中国
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Quick Overview for ARF1 抗体 (AA 174-180) (ABIN6936427)

抗原

See all ARF1 抗体
ARF1 (ADP-Ribosylation Factor 1 (ARF1))

适用

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宿主

  • 80
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小鼠

克隆类型

  • 60
  • 53
单克隆

标记

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This ARF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

3F1
  • 抗原表位

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    AA 174-180

    特异性

    The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

    交叉反应 (详细)

    Human. Mouse. Rat.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.

    亚型

    IgG2a kappa
  • 应用备注

    Known_Application: ELISA (For coating, order antibody without BSA),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa cells. Kidney, Liver, Brain or Breast.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    ARF1 (ADP-Ribosylation Factor 1 (ARF1))

    别名

    ARF1

    背景

    ADP-ribosylation factor 1, ARF1,ARF1 (Golgi Apparatus Marker)
    Cellular localisation: Cytoplasmic (Golgi Apparatus)

    分子量

    20kDa

    基因ID

    375, 286221

    UniProt

    P84077

    途径

    Transition Metal Ion Homeostasis, Inositol Metabolic Process
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