ARF1 抗体 (AA 174-180)
Quick Overview for ARF1 抗体 (AA 174-180) (ABIN6936427)
抗原
See all ARF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 174-180
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特异性
- The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
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交叉反应 (详细)
- Human. Mouse. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Synthetic peptide SNQLRNQ, corresponding to C terminal amino acids 174-180 of Human ARF1.
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亚型
- IgG2a kappa
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Kidney, Liver, Brain or Breast.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- ARF1 (ADP-Ribosylation Factor 1 (ARF1))
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别名
- ARF1
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背景
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ADP-ribosylation factor 1, ARF1,ARF1 (Golgi Apparatus Marker)
Cellular localisation: Cytoplasmic (Golgi Apparatus) -
分子量
- 20kDa
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基因ID
- 375, 286221
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UniProt
- P84077
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途径
- Transition Metal Ion Homeostasis, Inositol Metabolic Process
抗原
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