PLAP 抗体
Quick Overview for PLAP 抗体 (ABIN6936400)
抗原
See all PLAP (ALPP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Purified human PLAP protein
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亚型
- IgG2b kappa
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anti-Placental Alkaline Phosphatase (ALPP) (AA 162-312) antibody
ALPP 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 小鼠 Monoclonal ALP-870 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (N-Term) antibodyALPP 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 小鼠 Monoclonal GM022 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 兔 Monoclonal ALPP-2899R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-350R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (N-Term) antibodyALPP 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (AA 20-143) antibodyALPP 适用: 人 WB, ELISA, IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (AA 534-792) antibodyALPP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Frozen & Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)Optimal dilution for a specific application should be determined.
Positive_Control: JEG-3 or HepG2 cells. Placenta or seminoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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别名
- ALPP
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背景
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Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, ALP, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP-1, PLAP1,Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)
Cellular localisation: Cytoplasmic and cell surface -
分子量
- 70kDa
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基因ID
- 250, 284255
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UniProt
- P05187
抗原
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