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CD20 抗体 (PE)

This anti-CD20 antibody is a 小鼠 单克隆 antibody detecting CD20 in FACS, IF 和 T. Suitable for 人.
产品编号 ABIN6935147
发货至: 中国
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Room 801-803
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Quick Overview for CD20 抗体 (PE) (ABIN6935147)

抗原

See all CD20 (MS4A1) 抗体
CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD20 antibody is conjugated to PE

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

克隆位点

B9E9
  • 特异性

    Recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

    纯化方法

    Purified by Protein A/G

    免疫原

    Lymphoblastoid cell line Daudi

    亚型

    IgG2a kappa
  • 应用备注

    Positive Control: Daudi, Raji and U266 and human lymphocytes.

    Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    100 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA and 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

    别名

    MS4A1

    分子量

    33-37kDa

    基因ID

    931

    UniProt

    P11836
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