Vimentin 抗体 (CF®488A)
Quick Overview for Vimentin 抗体 (CF®488A) (ABIN6934573)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
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无交叉反应
- 小鼠, 大鼠
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纯化方法
- Purified by Protein A/G
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免疫原
- Recombinant human vimentin protein
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亚型
- IgG1 kappa
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应用备注
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Positive Control: Jurkat cells, Sarcomas, Melanomas.
Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 100 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA and 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Vimentin (VIM)
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别名
- VIM
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分子量
- 57-60kDa
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基因ID
- 7431
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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