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Vimentin 抗体 (CF®488A)

This anti-Vimentin antibody is a 小鼠 单克隆 antibody detecting Vimentin in IF, FACS 和 T. Suitable for 人, Pig, Cow, 小鸡, 犬, Cat 和 山羊.
产品编号 ABIN6934573
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (CF®488A) (ABIN6934573)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, Pig, Cow, 小鸡, 犬, Cat, 山羊

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is conjugated to CF®488A

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Test (T)

克隆位点

VM452
  • 特异性

    This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

    无交叉反应

    小鼠, 大鼠

    纯化方法

    Purified by Protein A/G

    免疫原

    Recombinant human vimentin protein

    亚型

    IgG1 kappa
  • 应用备注

    Positive Control: Jurkat cells, Sarcomas, Melanomas.

    Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    100 μg/mL

    缓冲液

    10 mM PBS with 0.05 % BSA and 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Vimentin (VIM)

    别名

    VIM

    分子量

    57-60kDa

    基因ID

    7431

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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