GAPDH 抗体
Quick Overview for GAPDH 抗体 (ABIN6923148)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- conc. tissue culture supernatant
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亚型
- IgM
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibodyVerified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibodyGAPDH 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 斑马鱼 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibodyGAPDH 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- The antibody solution can be used at dilutions of at least 1:100 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000 or lower. It is very useful as a loading control for quantitative western blotting.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Supplied as purified antibody at 1mg/mL in PBS and glycerol (1:1) with 5mM NaN3
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4°C short term or -20°C long term.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- Glyceraldehyde 3-Phosphate Dehydrogenase
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背景
- Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. The HGNC name for this protein is GAPDH.
抗原
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