Cadherin 抗体 (AA 841-882)
(2 references)
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北京 101111
Quick Overview for Cadherin 抗体 (AA 841-882) (ABIN687682)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 841-882
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交叉反应
- 人, 小鼠
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预测反应
- Rat,Dog,Cow,Pig,Horse,Chicken
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human E-cadherin
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亚型
- IgG
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anti-Cadherin (AA 739-767), (C-Term) antibody
适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin (AA 833-882) antibody适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 -
限制
- 仅限研究用
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- by
- Flow Cytometry & Cell Separation Facility, Purdue University
- No.
- #029742
- 日期
- 2014.06.30
- 抗原
- Lot Number
- 131101
- Method validated
- Flow Cytometry
- Positive Control
- MCF7 cells (high expression)
- Negative Control
- SH-SY5Y cells (low expression)
- Notes
- Signal was observed in the positive control cells. There was some non-specific staining observed with the isotype and secondary-only antibody controls which was lower than the signal from the anti-E-cadherin antibody. Low signal was observed in the low expressing (negative control) cells as expected.
- Primary Antibody
- Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
- Catalog number: ABIN687682
- Lot number: 131101
- Secondary Antibody
- Antibody: Goat anti-rabbit IgG-Alexa 647
- Full Protocol
- Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
- Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
- Cells were incubated for 30 min on ice.
- Labeled cells were washed twice in PBS containing 0.5% BSA.
- Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
- Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
- Labeled cells were washed twice in PBS containing 0.5% BSA.
- Propidium Iodide (PI) was added to discern live cells from dead cells.
- Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
- Experimental Notes
- - The data displayed is gated on PI negative cells.
生效 #029742 (Flow Cytometry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1. Histogram of MCF7 cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).]()
Figure 1. Histogram of MCF7 cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).
![Figure 2. Histogram of SH-SY5Y cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).]()
Figure 2. Histogram of SH-SY5Y cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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: "RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-?B signaling pathway in trophoblast cells." in: Acta histochemica, Vol. 116, Issue 5, pp. 911-9, (2014) (PubMed).
: "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 41, (2014) (PubMed).
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: "RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-?B signaling pathway in trophoblast cells." in: Acta histochemica, Vol. 116, Issue 5, pp. 911-9, (2014) (PubMed).
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- Cadherin
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背景
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Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1
Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
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基因ID
- 999
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UniProt
- P12830
抗原
-
