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EPO 抗体 (AA 101-193)

Name: EPO 抗体 (AA 101-193)
SKU: ABIN679718
Availability: OutOfStock

This anti-EPO antibody is a 兔多克隆 antibody detecting EPO in WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 和 DB. Suitable for 人和大鼠. This Primary Antibody has been cited in 4+ publications.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-EPO Polyclonal Antibody, Unconjugated (ABIN679718) at 1:200 followed by conjugation to the secondary antibody and DAB staining (EPO 抗体 (AA 101-193))

IHC

Rat lung lysates probed (ABIN679718) at 1:200 in 4 °C. (EPO 抗体 (AA 101-193))

SDS

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with EPO Polyclonal Antibody, Unconjugated ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (EPO 抗体 (AA 101-193))

IHC (p)

3 images

(4 references)

产品编号 ABIN679718

发货至: 中国

Datasheet as PDF

Quick Overview for EPO 抗体 (AA 101-193) (ABIN679718)

抗原

See all EPO 抗体

EPO (Erythropoietin (EPO))

适用

人, 大鼠

宿主

兔

克隆类型

多克隆

标记

This EPO antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Discover our top product EPO Primary Antibody

抗原表位
48

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1
AA 101-193

交叉反应

人, 大鼠

预测反应

Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Erythropoietin

亚型

IgG
anti-Erythropoietin (EPO) (AA 28-193) antibody
EPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Erythropoietin (EPO) (AA 28-193) antibody
EPO 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C3 unconjugated

anti-Erythropoietin (EPO) antibody
EPO 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Erythropoietin (EPO) (AA 28-162) antibody
EPO 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal EPO-1368 unconjugated

anti-Erythropoietin (EPO) (AA 28-162) antibody
EPO 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal EPO-1367 unconjugated

anti-Erythropoietin (EPO) (AA 20-48) antibody
EPO 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Erythropoietin (EPO) (AA 28-191) antibody
EPO 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Erythropoietin (EPO) antibody
EPO 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Erythropoietin (EPO) (AA 28-193) antibody
EPO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G7 unconjugated

anti-Erythropoietin (EPO) antibody
EPO 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 4F11 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
dot-blot()

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Desharnais, Naud, Ayotte: "Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system." in: Drug testing and analysis, Vol. 10, Issue 11-12, pp. 1698-1707, (2019) (PubMed).

Ichii, Nakamura, Irie, Kouguchi, Nakamura, Nakamura, Sato, Yokoyama, Horino, Sunden, Elewa, Kon: "Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes." in: Histochemistry and cell biology, Vol. 146, Issue 3, pp. 351-62, (2016) (PubMed).

Li, Chen, Shao, Tang, Chen, Chen, Xu: "Oxidative stress induces the decline of brain EPO expression in aging rats." in: Experimental gerontology, Vol. 83, pp. 89-93, (2016) (PubMed).

DeNiro, Al-Mohanna: "Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment." in: PLoS ONE, Vol. 9, Issue 7, pp. e101602, (2014) (PubMed).
抗原

EPO (Erythropoietin (EPO))

别名

EPO

物质类

Hormone

背景

Synonyms: EP, MVCD2, Erythropoietin, EPO
Background: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

基因ID

2056

UniProt

P01588

途径

JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity