LRP5 抗体 (C-Term)
Quick Overview for LRP5 抗体 (C-Term) (ABIN6757408)
抗原
See all LRP5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species cross-reactivity: Mouse.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide derived from the C-terminal region of the human LRP5 protein. This intracellular region is identical to the corresponding mouse and rat LRP5 amino acid sequences.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IF (2 - 3 μg/mL), WB (1 - 3 μg/mL)
Usage: Suitable for use in Immunofluorescence and Western Blot. Immunofluorescence: 2-3 μg/mL, Note: For optimal results in immunofluorescence assays, cells should be fixed in 4 % paraformaldehyde/0.5 % Triton X-100/PBS for 10 minutes, permeabilized with 0.5 % Triton X-100/PBS for 5 minutes, and blocked with 5 % BSA/PBS for 30 minutes. Western Blot: 1-3 μg/mL, Single band at ~200kD. Positive control: Mouse LRP5-pSEC-tag-transfected 293T cells, MCF-7 human breast adenocarcinoma, T47D human breast ductal carcinoma, and NIH-3T3 mouse embryo fibroblast lysates. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide, 40 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))
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别名
- LRP5
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背景
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Name/Gene ID: LRP5
Synonyms: LRP5, EVR1, Exudative vitreoretinopathy 1, LRP7, OPTA1, OPPG, OPS, LR3, LRP-5, VBCH2, BMND1, EVR4 -
基因ID
- 4041
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UniProt
- O75197
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途径
- WNT signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation
抗原
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