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Nanog 抗体 (AA 135-184)

This anti-Nanog antibody is a 兔 多克隆 antibody detecting Nanog in WB. Suitable for 人, Cow, 绵羊 和 猴. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN6746019
发货至: 中国

Quick Overview for Nanog 抗体 (AA 135-184) (ABIN6746019)

抗原

See all Nanog (NANOG) 抗体
Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

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人, Cow, 绵羊, 猴

宿主

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克隆类型

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多克隆

标记

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This Nanog antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

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    AA 135-184

    特异性

    Human Nanog

    预测反应

    Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Pig (92%) Sheep, Bovine (85%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide located between aa135-184 of human NANOG (Q6NSW7, NP_079141). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Dog, Cat, Horse, Pig, Opossum (92%), Sheep, Bovine, Rabbit (85%).

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Distilled water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS with 2 % sucrose

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Ku, Meier: "Phosphorylation of paxillin via the ERK mitogen-activated protein kinase cascade in EL4 thymoma cells." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11333-40, (2000) (PubMed).

    Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, (1999) (PubMed).

    Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany, Sun, Pisick, Hallek, Ernst: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, (1995) (PubMed).

  • 抗原

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    别名

    NANOG

    背景

    Name/Gene ID: NANOG

    Synonyms: NANOG, Nanog homeobox, NANOGP8, HNanog, Homeobox protein NANOG

    基因ID

    79923

    NCBI登录号

    NP_079141

    UniProt

    Q9H9S0

    途径

    Stem Cell Maintenance
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