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GTPBP10 抗体 (AA 254-303)

This anti-GTPBP10 antibody is a 兔 多克隆 antibody detecting GTPBP10 in WB, IHC 和 IHC (p). Suitable for 人, 小鼠, 大鼠, 兔, 马, Pig, Hamster 和 猴.
产品编号 ABIN6741598
发货至: 中国

Quick Overview for GTPBP10 抗体 (AA 254-303) (ABIN6741598)

抗原

See all GTPBP10 抗体
GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

适用

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人, 小鼠, 大鼠, 兔, 马, Pig, Hamster, 猴

宿主

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克隆类型

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多克隆

标记

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This GTPBP10 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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    AA 254-303

    品牌

    IHC-plus™

    特异性

    Human GTPBP10

    预测反应

    Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig (100%) Dog, Guinea pig (92%) Chicken (85%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide located between aa254-303 of human GTPBP10 (A4D1E9, NP_149098). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Rabbit, Horse, Pig, Opossum, Armadillo, Lizard, Stickleback (100%), Dog, Bovine, Bat, Guinea pig (92%), Salmon (90%), Turkey, Zebra finch, Chicken, Platypus (85%), Zebrafish (84%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Distilled Water.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS with 2 % sucrose

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原

    GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

    别名

    GTPBP10

    背景

    Name/Gene ID: GTPBP10

    Synonyms: GTPBP10, DKFZP686A10121, ObgH2, Protein obg homolog 2, UG0751c10, GTP-binding protein 10

    基因ID

    85865

    NCBI登录号

    NP_149098

    UniProt

    A4D1E9
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